PPARG and obesity due to melanocortin 4 receptor deficiency: Western blot analysis showed that a clinical trial test material made of ES extract reduced the protein expression of C/EBP-α, PPAR-γ, and aP2 to inhibit adipocyte differentiation while promoting the expression of UCP-1 and CPT-1 in adipocytes to increase energy expenditure and play a key role in improving obesity [38].