In this study, an in vitro model generated by coculturing mouse dorsal root ganglion (DRG) neuronal cells and PC cells, and an in vivo PNI model were applied to investigate the function and underlying mechanisms of NGF/TrkA signaling in the progression and pathogenesis of PNI, contributing to the development of therapeutic regimens for PC. This evidence concerns the gene NGF and pachyonychia congenita.