To further evaluate whether the GD2 expression level was mediated by B3GALT4, we established stable NB cell lines with B3GALT4 knockdown (sh-B3GALT4) and B3GALT4 overexpression (B3GALT4) NB cell lines (Fig. 1J and K) and analyzed the expression level of GD2 in these cells using a flow cytometry assay. This evidence concerns the gene B3GALT4 and neuroblastoma.