To confirm the above-mentioned data and to shed new light on the transcriptional changes that HECs adopt to limit CRC trans-sinusoidal migration, we performed RNA-seq analyses on CD31+ endothelial cells isolated from the liver of Ifnar1fl/fl or VeCadIfnar1_KO mice 7 days after NaCl or IFNα treatment (Figure 6—figure supplement 1A). The gene discussed is IFNA1; the disease is colorectal carcinoma.