MAPK1 and Anal fistula: As the anal fistula model established by small animals failed to explain the pathogenesis and related mechanisms of the anal fistula, we constructed the landrace pigs anal fistula model and then observed the effects of CGF treatment on the secretion of inflammatory factors, angiogenesis, fibroblast activation, ECM degradation, activation of the ERK pathway, and microbial population in pig fistula tissues to clarify the mechanism of CGF treatment for anal fistula healing.