To verify the reliability of our model genes, the relationship between COX10-AS1 and pyroptosis was verified by U87 glioma cells in vitro, and it has been confirmed that COX10-AS1 was an oncogenic role involved in cell proliferation and invasion of glioma cells via affecting tumor cell growth by CXO10-AS1/miR-641/E2F6 feedback loop [18] and COX10-AS1/miR-361-5p/ACTG1 [19], but no studies have tested its correlation with pyroptosis. Here, E2F6 is linked to central nervous system cancer.