In tumor cells, as illustrated in Fig. 8, RAB22A binds to PI4K2A that generates PI4P, which recruits the Atg12–Atg5–Atg16L1 complex to induce LC3-II anchoring on the ER-derived membrane to form the RAB22A-mediated non-canonical autophagosome. The gene discussed is ATG16L1; the disease is neoplasm.