Furthermore, according to the ELISA assay of HMGB1 expression in Fig. 5f, compared with the PBS group, VNP@Gel and GD/EI-NP@Gel slightly increased the HMGB1 expressions in the tumor tissues, while the VNP-GD@Gel displayed a threefold increase of HMGB1 expression. The gene discussed is HMGB1; the disease is neoplasm.