In this study, we presented a VNP bacteria-based GSDMD protein delivery system for in vivo antitumor treatment, in which VNP could efficiently shuttle GSDMD to the intracellular compartment of tumor cells where flagella on VNP-activated capapase-1 to further cleave delivered GSDMD to N-terminal domain for effective pore-forming and pyroptosis. Here, GSDMD is linked to neoplasm.