PDC and infection: We also quantified pDC/CD169+ MP contact durations using a Kiss and Run algorithm (Imaris) as a measure of their possible interactions in chimeric mice and found that WT pDC significantly increased their contact times with the MP during infection (from ~2 to 16 min) whereas Tlr7−/y pDC interacted with the MP for comparable amounts of time (~4–5 min) whether mice were infected or not (Figure 3G).