HSPD1 and Menkes disease: In the context of MD, however, no evidence of changes in mitochondrial content, as evaluated through immunoblotting for the mitochondrial proteins succinate dehydrogenase A and heat shock protein 60 (Supplemental Figure 3B), or blood vessel density, as evaluated by CD31+ staining (Supplemental Figure 3C), were observed as a result of NOX4 ablation.