To explore whether the Shh pathway mediates the antitumor effect of metformin on medulloblastoma, after the treatment of Daoy cells with SAG (100 nm) and metformin (3 mm), we found that the SAG can attenuate the inhibitory effect of metformin on Gli-1 (Figures 6A and B). Here, GLI1 is linked to medulloblastoma.