It was found that the reduced expressions of SIRT1, LXR, and FXR were associated with increased levels of lipid, alanine transaminase (ALT), alkaline phosphatase (ALP), and aspartate transaminase (AST) in rats with NAFLD, along with a significantly increased percentage of hepatocyte apoptosis (Hajighasem et al., 2013). This evidence concerns the gene NR1H4 and metabolic dysfunction-associated steatotic liver disease.