The CIBERSORT algorithm was utilized to evaluate the discrepancies in the levels of 22 populations of immune cells in these CRC tumors, revealing that there were high levels of M0 macrophages, CD8+ T cells, naïve B cells, and resting memory CD4+ T cells in cluster 2 patient tumors (Figure 2(e)). The gene discussed is CD8A; the disease is colorectal carcinoma.