Out of the entire retrospective cohort of 616 patients with ACTH-dependent Cushing’s syndrome (Tübingen, n=167 (27%); Munich, n=149 (24%); Vienna, n=118 (19%); Würzburg, n=108 (18%); Milan, n=47 (8%); Berlin, n=27 (4%)), 556 (90%) underwent a CRH stimulation test and/or an overnight 8 mg DST. The gene discussed is POMC; the disease is Cushing syndrome due to macronodular adrenal hyperplasia.