We investigated whether the differences in zDHHC5 expression and substrate palmitoylation identified early in cardiac hypertrophy persists in HF by analysing two experimental models (rabbit and pig) of myocardial infarction (MI) induced HF, as well as samples from ischaemic human HF patients (classified as reduced ejection fraction, details in Supplementary Table S1). This evidence concerns the gene ZDHHC5 and cardiac hypertrophy.