To further confirm the expression level of these regulators in colorectal cancer cell line and normal colonic epithelial cell line, we selected three most significantly differentially expressed regulators between tumor and normal tissues, DNMT3B (writer), TET2 (eraser), and UHRF1 (reader), to compare their mRNA expression in HCT116 and NCM460 cells. The gene discussed is TET2; the disease is neoplasm.