Interestingly, compared to normal tissues, we found that many regulators with amplified CNV had a markedly higher expression level in colon cancer tissues (e.g., DNMT1 and DNMT3B), and vice versa (e.g., MBD2 and ZBTB4), suggesting that the alteration of CNV might be the prominent factor resulting in the abnormal expression of DNA methylation regulators (Figures 1C,D). This evidence concerns the gene ZBTB4 and colonic neoplasm.