CDCP1 and lung adenocarcinoma: To quantify internalisation, TKCC05 PDAC cells stably silenced for CDCP1 and shRNA control TKCC05 cells (Figure 2B, left top), and lung adenocarcinoma A549 cells stably over-expressing CDCP1 and vector control A549 cells (Figure 2B, left bottom), were treated with antibodies labelled with a Fab-fluor pH-sensitive dye then imaged by time-lapse microscopy to monitor accumulation of cellular fluorescence as a measure of antibody internalization.