ZDHHC6 and hepatocellular carcinoma: To further elucidate the roles of Zdhhc6 in HCC development, Zdhhc6 was depleted (Zdhhc6-/-) in mice using CRISPR/Cas9 (Figure S3), which also resulted in a nearly complete abolition of AEG-1 palmitoylation in mouse liver (Figure 4A).