As shown earlier, DEN-induced HCC model was incorporated in Zdhhc6-/- mouse (Figure 4B), similarly, all mice developed typical HCC accompanying with severe fibrosis (Figure 4C-D), and strikingly, parameters as the liver weight/body weight ratio, tumor number, and maximum tumor diameter were significantly elevated in Zdhhc6-/- mice as compared with WT mice (Figure 4E-G), while no spontaneous liver injury or malignancies were observed in both genotypes on normal chow diet (Figure 4C-D). Here, ZDHHC6 is linked to hepatocellular carcinoma.