CDK20 and nonpapillary renal cell carcinoma: In addition, the results confirmed that CCRK expression was decreased in the miR-335-5p mimic group compared with the miR-NC group by western blotting; in contrast, the CCRK expression was notably recovered in the miR-335-5p mimic + LV-CCRK group after ccRCC cells retransfected with CCRK vector (Figures 6(g) and 6(i)).