Figure 1(a) was the correction diagram of removing batch. The PCA (principal component analysis) showed that the tumor group and normal group could be well districted (Figure 1(b)). Moreover, we observed a good correlation between groups and genetic characteristics (Figure 1(c)). A total of 429 upregulated genes and 418 downregulated genes (including LIMS2) were found between tumor and normal groups, as shown by the volcano plot (Figure 1(d)). These results indicated that LIMS2 was downregulated in OS. The gene discussed is LIMS2; the disease is neoplasm.