We further verified SPP1 in PCa and BPH samples by using the ELISA method, finding that SPP1 did not increase in the early stage of PCa, while significantly higher concentration was observed in the AG group when combining the GS 7 (3 + 4) group with GS 6 and the GS 7 (4 + 3) group with GS 8, 9, which was consistent with the MS analysis. This evidence concerns the gene SPP1 and posterior cortical atrophy.