A volcano map was made for significantly differential proteins with screening criteria of Q value< 0.05 and FC> 1.5, showing that L-selectin, T-complex protein 1 subunit epsilon (CCT5), and gamma-glutamyltransferase light chain 2 (GGTLC2) were significantly increased in Gleason 6 when compared with BPH, while glutamate oxaloacetate transaminase 2 (GOT2), pancreatitis-associated protein 1 (REG3A), and caspase recruitment domain-containing protein 5 (PYCARD) were significantly decreased in Gleason 6 (Figure 4A). The gene discussed is GGTLC2; the disease is benign prostatic hyperplasia.