Then, through various cell biology and molecular biology experiments, it was established that DNAAF5 can be used as a scaffold protein to recruit the deubiquitination protein (USP39) and the rate-limiting enzyme (PFKL) of glucose metabolism to improve protein stability of the latter, thereby enhancing glycolysis in HCC cells to accelerate their malignant progressions. Here, PFKL is linked to hepatocellular carcinoma.