It was revealed that DNAAF5 potentially interacts with PFKL (phosphofructokinase, liver type) protein, which encodes the pivotal enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, key step glycolysis, which led us to explore whether DNAAF5 plays an important role in affecting glycolysis, the key biological process in hepatocellular carcinoma, by interacting with PFKL. Here, DNAAF5 is linked to hepatocellular carcinoma.