FGFR1 and cancer: To investigate whether overproduction of FGFR1 can make cancer cells less sensitive to drugs that interfere with tubulin polymerization, we measured the viability of cells in the U2OS sublines: a control cell line, transfected with empty pcDNA3.1 vector (U2OS), cells stably transfected with FGFR1 wild-type (U2OSR1) and the kinase-dead mutant of FGFR1 (U2OSR1-K514R) after 48 h of treatment with 5 nM taltobulin (TLT), 20 nM paclitaxel (PTX), and 10 nM vincristine (VCR), using the alamarBlue Cell Viability Reagent (Figure 1A).