Figures 10A–E showed that, based on GTEx and TCGA databases, all five HLMRGs were differentially expressed between BC and normal samples. DARS2, ESRP1, SLC2A1, and TH were increased in BC, whereas MAFF was increased in normal tissues. Survival analysis indicated that high expression of DARS2, ESRP1, SLC2A1, and TH were related to poor prognosis, while high expression of MAFF was linked to better prognosis (Figures 10F–J). Here, DARS2 is linked to breast cancer.