ASIC1 and pulmonary arterial hypertension: To determine the specific role of ASIC1a in PAEC and PASMC remodeling in pulmonary hypertension, we generated mice with either EC (TekCre-Asic1afl/fl) or conditional SM (MHCCreER-Asic1afl/fl) specific deletion of Asic1a. As demonstrated previously in Asic1a+/+ and Asic1a−/- mice (Nitta et al., 2014), ASIC1 was detected as punctate fluorescence within the PASMCs and PAECs from Asic1afl/fl and MHCCreER-Asic1afl/fl (without TAM-induced Cre recombinase) mice (Figures 3A,B).