To determine the mechanism underlying chemotherapy-induced CD47 upregulation in osteosarcoma, we treated different osteosarcoma cell lines with doxorubicin using a wide range of treatment time and doses, which surprisedly failed to obviously alter CD47 expression in the osteosarcoma cancer cells (Supplementary Fig. 2a–h), suggesting that doxorubicin-induced CD47 upregulation in osteosarcoma cells is tumor microenvironment-dependent. This evidence concerns the gene CD47 and osteosarcoma.