Thus, overexpression of REDD1KKAA did not induce NF-κB activation, preadipocyte differentiation, and cytokine production in vitro; similar to Redd1−/− mice, Redd1KKAA mice presented restrained obesity-induced characteristics, including proinflammatory cytokine production and insulin resistance. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.