Interestingly, naive CD4+ and CD8+ T cells displayed increased expression of the memory-associated marker CD45RO after 48 and 72 h of coculture with MDA-MB-468 tumor cells in the presence of DuoBody-CD3x5T4 (Fig 1F and S4C), indicating a shift of the naive T-cell subsets toward a memory-like phenotype. Here, CD8A is linked to neoplasm.