Similar to the results of the OFT, male TG mice showed an increased anxiety-like behavior as evidenced by a reduced number of entries into the open arms, a decreased time in the open arms, and a decreased distance traveled in the open arms (Fig 4I) that was accompanied by a decreased distance traveled in the closed arms and an increased time freezing (Fig 4K) which was reversed in TGxGfKO mice, indicating an involvement of GFRAL signaling in inducing anxiety-like behavior in male mice. This evidence concerns the gene GFRAL and Anxiety.