We found that the levels of AST [Fig. 2A, ANOVA: F(3, 8) = 6.427, P = 0.007], ALT [Fig. 2B, ANOVA: F(3, 9) = 26.378, P < 0.001[, DBIL [Fig. 2C, ANOVA: F(3, 12) = 2.762, P = 0.026], and TBA [Fig. 2D, ANOVA: F(3, 9) = 6.884, P = 0.008] were significantly increased after worm infection in wild-type mice, compared with normal control mice, suggesting C. sinenesis infection caused severe hepato-biliary injuries. This evidence concerns the gene GPT and helminthiasis.