PAK1 and pancreatic adenocarcinoma: The WNK2 knockout cellular models displayed higher phospho-PAK1/2 and phospho-ERK1/2 levels, implying that WNK2 depletion promoted the activation of the MAPK pathway and in agreement with previous results in HeLa and HT-29 cells,40 41 pancreatic adenocarcinoma tissue38 and in hepatocarcinoma cell lines.43 All tested WNK2 variants showed the same tendency, with a prominent WNK2 malfunction detected for the p.Pro702Leu variant, which displayed the most significant phosphorylation levels of both PAK1/2 and ERK1/2 in WNK2KO2 and WNK2KO7 clones.