In addition to R-66S gRNA, we applied SMRT-seq with UMI to quantify both small and large gene modifications in SCD HSPCs induced by three other CRISPR gRNAs, including R-02 (33), SD-02 (27), and BCL11A gRNAs designed for treating SCD or β-thalassemia (28). The gene discussed is BCL11A; the disease is Schnyder corneal dystrophy.