The PC-3 cell line was chosen because YTHDF2 has shownto be involved in cancer development and its expression level is correlatedwith the poor prognosis of prostate cancer patients.20 To assess which concentration could be feasible to performfurther experiments, we evaluated the molecule’s toxicity bytreating cells with different concentrations for 24, 48, and 72 h.IC50 values were calculated for each time point (Figure 3A), and the compound’stoxicity increased with increasing hours of treatment. Here, YTHDF2 is linked to prostate cancer.