To clarify whether miR-585-5p regulates GC cell growth and metastasis inhibition through MITF, we adopted an MITF antagonism-of-function strategy employing the MITF expression vector without miR-585-binding sites in miR-585-5p-co-expressing AGS and BGC823 cultures and found that MITF overexpression reverses the in vitro inhibitory effect of miR-585-5p on proliferative/metastatic properties in GC (Figure 4E). This evidence concerns the gene MITF and gastric cancer.