In this case report, we identified a novel nonreciprocal <i>ALK</i> fusion, namely, <i>junctional sarcoplasmic reticulum protein 1</i> (<i>JSRP1</i>) intergenic region-<i>ALK</i> fusion (Jintergenic: A20) <i>via</i> next-generation sequencing in a female patient initially diagnosed with stage IV B lung adenocarcinoma. This evidence concerns the gene JSRP1 and lung adenocarcinoma.