MYOC and open-angle glaucoma: To evaluate whether pathogenic MYOC mutations cause cellular oxidative stress, we transfected COS-7 cells with two secreted variants (one pathogenic variation L215Q and one neutral polymorphism V329M) and two nonsecreted mutants (G367R and P370L that were reported to cause severe POAG phenotypes), and H2O2 sensitivity, ROS generation and mitochondrial function were analyzed.