Glytrexate, (S)-2-{2-[2-(2-amino-4-oxo-4,7-dihydro-3H-pyrrolo [2,3-d]pyrimidin-6-yl)-acetylamino]-acetylamino}-pentanedioic acid (1), was one of a series of compounds identified as a multitarget inhibitor of TS, GARFTase, and AICARFTase, and exhibited antiproliferative effects in a series of tumor cell lines including KB, SW620, and MCF7 cells (Xing et al., 2017). This evidence concerns the gene TYMS and neoplasm.