TYMS and neoplasm: In our previous study, possible anticancer drugs targeting both thymidylate and purine nucleotide biosynthesis were investigated, and glytrexate was one of a series of compounds identified as a multitarget inhibitors of TS, GARFTase, and AICARFTase and found to have antiproliferative effects in a series of tumor cell lines including KB, SW620, and MCF7.