TGFB1 and non-small cell lung carcinoma: In non-small cell lung cancer cells transfected with a pMRX-IP-green fluorescent protein (GFP)-LC3-red fluorescent protein (RFP)-LC3ΔGly construct, TGFβ decreased the GFP/RFP ratio, which verified that TGFβ upregulated autophagic flux (Trelford and Guglielmo, 2020).