This complex was delivered in the 3D co-culture TME model of 4T1 mouse breast cancer cells and Balb/c 6–8 weeks old female mouse-derived tumour associated-macrophages for invasion arrest investigation that successfully switched-off the expression of RICTOR (Fig. 6a–c) resulting 75–85% macrophages polarization towards pro-inflammatory/growth-inhibition during breast carcinoma liver metastasis (Fig. 6d and e) [243]. This evidence concerns the gene RICTOR and breast carcinoma.