TCN2 and infection: Two sera panels were used: (i) sera from C57BL6 mice experimentally infected with TcI, TcII, and TcVI strains, collected during acute and chronic phases of T. cruzi infection to assess the variability in host recognition in the acute and early chronic stages of the infection (Fig. 5 and Table S2), and (ii) sera from chronic Chagasic patients to assess variability in host recognition in the long-lasting chronic human infection (Fig. 6).