TNF and Wilson disease: Similarly, we isolated splenocytes from SC-, WD-, and KD-fed mice and found no difference between homeostatic inflammation of splenocytes between diets, but a significantly enhanced expression of Tnf in the splenocytes of KD-fed mice, and not WD-fed mice, challenged with LPS (2 hr) compared to splenocytes from SC-fed mice (Figure 2—figure supplement 1E, F).