Through integrative analysis of single-cell RNA sequencing data of immune cells from moderate and severe COVID-19 patients, the expression levels of pyroptosis-associated markers NLRC5, caspase-1, caspase-4, GSDMD, Ninjurin 1 (NINJ1), IL-1β and IL-18 were found to be significantly higher in severe patients than moderate patients 137. The gene discussed is GSDMD; the disease is COVID-19.