In a human osteosarcoma cell line with DNA damage induced by ionizing radiation, EZH2 was recruited to the DNA double-strand damage site marked by γ-H2AX, and Suz12 and EED were also recruited to DNA damage sites as other components of the PRC2 complex; however, with PARP inhibitor treatment, EZH2 was unable to localize at the DNA double-strand damage site, suggesting that this process is PARPdependent. Here, EZH2 is linked to osteosarcoma.