Over time the antibody titers to both WU and OM viruses waned significantly, and at week 41 the animals were boosted with a third vaccination, either with the original WU-vaccine (vaccination regime WU-WU-WU) or an updated OM-vaccine that incorporated spike protein antigen from the OM virus (regime WU-WU-OM). The gene discussed is CHMP5; the disease is ocular melanoma.