Given the well-documented role of these CRC genes in maintaining the rapidly proliferating phenotype of neuroblastoma cells (Boeva et al., 2017; Durbin et al., 2018) and the reduced proliferation we saw after ASCL1 deletion, we then looked to see if ASCL1 KO was affecting the level and/or activity of PHOX2B and GATA3 proteins using western blotting. This evidence concerns the gene ASCL1 and neuroblastoma.