Consistent with our observation that ASCL1 KO has little effect on PHOX2B, GATA3, and MYC/MYCN gene transcript levels (Figure 5A), ATAC-seq analysis showed that ASCL1 deletion had little effect on chromatin accessibility at the CRC genes (Figure 5B, Supplementary Figure S5). This evidence concerns the gene MYCN and colorectal carcinoma.