Reduced binding of these factors is likely to contribute to the reduced proliferation of ASCL1 KO cells, particularly in the case of MYCN/MYC, whose role in driving proliferation in neuroblastoma is very well documented (Huang and Weiss 2013; Zimmerman et al., 2018; Otte et al., 2021). The gene discussed is ASCL1; the disease is neuroblastoma.