Thirty-six pyroptosis genes exhibited significantly higher transcriptional levels in HCC, including APIP, BAK1, BAX, CASP3, CASP4, CASP6, CASP8, CASP9, CHMP2A, CHMP2B, CHMP3, CHMP4A, CHMP4B, CHMP4C, CHMP6, CHMP7, CYCS, DFNA5, DHX9, GPX4, GSDMB, GSDMC, GSDMD, HMGB1, IL1A, IRF2, NLBP1, NLRP9, NOD1, NOD2, PLCG1, PRKACA, PYCARD, SCAF11, TIRAP, and TP53. The gene discussed is HMGB1; the disease is hepatocellular carcinoma.