The remarkable differences in protein levels of NLRP3, ASC, cleaved caspase-1, GSDMD-N, caspase-11, and mature IL-1β between the NS+I/R group and 4-PBA+I/R group again testified that renal I/R-generated pyroptosis could be regulated by ER stress in mice (Figures 4(i) and 4(j)). This evidence concerns the gene CASP1 and psychosocial stress measurement.