In addition, the positive rates of WT1-AS, RIOK1 IgG (18.2% vs. 5.3%, p = 0.008; 15.9% vs. 5.3%, p = 0.025) and PIGP, TCEAL6, GPBP1_frag IgA (20.5% vs. 5.3%, p = 0.001; 18.2% vs. 3.8%, p = 0.002; 15.9% vs. 5.3%, p = 0.013) were significantly higher in IBS patients with severe abdominal pain/discomfort than HCs. This evidence concerns the gene WT1 and irritable bowel syndrome.