The positive rates of RIOK1, WDR83, DTNBP1, WDR54 IgG (23.4% vs. 5.3%, p = 0.014; 14.5% vs. 5.3%, p = 0.031; 14.5% vs. 4.6%, p = 0.016; 12.9% vs. 2.3%, p = 0.003) and RIOK1, CACNG3, GAD1 IgA (17.7% vs. 4.6%, p = 0.003; 14.5% vs. 4.6%, p = 0.016; 11.3% vs. 2.3%, p = 0.008) were significantly higher in IBS patients with severe anxiety than HCs. Here, RIOK1 is linked to irritable bowel syndrome.