All SNPs followed HWE (p = 1.00), and it was observed that in RA individuals except for rs3764879 (TLR-8) allelic frequency of rs3764880 (TLR-8), rs5744080 (TLR-8), SCV000844945 (TLR-8), and SCV000844946 (TLR-8), polymorphic sites were not significantly varied in comparison to controls. This evidence concerns the gene TLR8 and rheumatoid arthritis.