To determine the generalizability of our findings, we used the single high dose 8 Gy to verify if radiation promotes vascular marker expression in multiple patient-derived gliomasphere lines that included the 3 major molecular subtypes: Proneural, Mesenchymal and Classical22, and both primary and recurrent IDH1-wild type and mutant GBM. This evidence concerns the gene IDH1 and glioblastoma.